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KMID : 0981820080280030239
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Korean Journal of Laboratory Medicine
2008 Volume.28 No. 3 p.239 ~ p.243
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Utility of In-House PCR for HLA-B27 Typing: Comparison of Concordance Rate between PCR Kit and In-House PCR
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Cho Sun-Young
Park Su-Yon
Lee Hee-Joo
Lee Kwang-Gil
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Abstract
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Background: Commercial kits of PCR method are widely used in HLA-B27 typing; however, their cost is relatively high. In this study, we evaluated the utility of an in-house PCR method by comparing it with that of a commercial kit.
Methods: HLA-B27 typing was done in 188 patients by using two PCR methods, AbsoluteTM HLAB27 PCR kit (Biosewoom, Korea) and an in-house PCR method. The primers used in the in-house method were prepared by Bioneer (Korea). Both PCR tests were done by Gene Amp PCR System 9600 (Perkin-Elmer Centus Corp., USA).
Results: The commercial kit and in-house PCR showed 100% concordance rate with each other in HLA-B27 typing. Of 188 patients tested 72 (38.3%) were positive and 116 (61.7%) were negative by the both tests. Of 62 patients with ankylosing spondylitis, 50 were positive (80.7%).
Conclusions: The in-house PCR is a reliable and cost-effective method and can replace or supplement
commercial kits for HLA-B27 typing.
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KEYWORD
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HLA-B27, In-house PCR, DNA typing
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